Biological Pick, Place, and Perfuse "BioP3" - Device to Engineer Thick Living Tissues with High Cell Density (Case 2220) (EA)

Principal Investigator:


Jeffrey Morgan, Ph. D

Professor – Molecular Pharmacology, Physiology and Biotechnology (MPPB)

Brown University

Providence, RI


Brief Description:


Approximately twenty-two people die in the United States everyday waiting for a life saving organ transplantation. The development of tissue-engineered organs to supplement our organ donor system is a novel solution to an unmet medical need. However, researchers have been struggling to overcome the challenges of creating large-scale, 3-D tissues due to a variety of bench top culturing limitations. Tissue perfusion techniques, needed for the delivery of nutrients to and removal of waste from tissues, is one of the greatest challenges researchers currently face and the reason why current 3-D tissue culturing techniques are limited to small scale tissues that rely on passive diffusion.


However, the innovative Biological Pick Place and Perfuse (BioP3) enables the creation of living, large-scale, 3-D, high cell density tissues through its novel, tissue building block stacking and perfusion technology. This device utilizes a specialized gripper head to pick up, align, and stack self-assembled 3-D micro tissues, containing millions of cells, upon one another layer-by-layer. These stacked micro tissues are then actively perfused with media through their aligned lumens, allowing the micro tissue building blocks to fuse into large, solid, living organisms. Thus, the BioP3’s exciting, cutting edge, layer-by-layer tissue stacking and perfusion techniques provide the perfect platform for the rapid production of large-scale, 3-D tissues.


Applications of this technology include organ-like tissue construction for organ transplants, specifically kidneys, livers and pancreases. This technology is suitable for anyone interested in the manufacture of large-scale, 3-D tissues for potential research or clinical use.




US Patent application 14/328,956 is pending.

Patent Information:
For Information, Contact:
Margaret Shabashevich,
Manager of Operations
Office of Industry Engagement & Commercial Venturing
Brown University
Jeffrey Morgan
Anubhav Tripathi
William Patterson Iii
John Murphy
Andrew Blakely
© 2020. All Rights Reserved. Powered by Inteum